Sedimentation Velocity Analysis of Heterogeneous Protein-Protein Interactions: Lamm Equation Modeling and Sedimentation Coefficient Distributions c(s)

Calculating sedimentation coefficient distributions by direct modeling of sedimentation velocity concentration profiles.

The c(s) method results in high-resolution sedimentation coefficient distributions by deconvoluting the effects of diffusion broadening. It is a direct model for sedimentation data covering the complete sedimentation process, based on finite element solutions of the Lamm equation, and algebraically accounts for the systematic noise structure encountered in transport experiments. A continuous se...

Characterizing protein-protein interactions by sedimentation velocity analytical ultracentrifugation.

This unit introduces the basic principles and practice of sedimentation velocity analytical ultracentrifugation for the study of reversible protein interactions, such as the characterization of self-association, heterogeneous association, multi-protein complexes, binding stoichiometry, and the determination of association constants. The analytical tools described include sedimentation coefficie...

Diffusion-deconvoluted sedimentation coefficient distributions for the analysis of interacting and non-interacting protein mixtures

Variation of sedimentation coefficient with rotor velocity.

The sedimentation coefficients of three proteins, which appeared homogeneous in the ultracentrifuge, have been determined at different rotor velocities. Two of the proteins, ovalbumin and human gamma-globulin, showed sedimentation coefficients that were approx. 10% higher at rotor velocities below 20000rev./min. than at 56100rev./min. The third protein, haemocyanin of Jasus lalandii, could not ...

Analytical ultracentrifugation: sedimentation velocity and sedimentation equilibrium.

Analytical ultracentrifugation (AUC) is a versatile and powerful method for the quantitative analysis of macromolecules in solution. AUC has broad applications for the study of biomacromolecules in a wide range of solvents and over a wide range of solute concentrations. Three optical systems are available for the analytical ultracentrifuge (absorbance, interference, and fluorescence) that permi...